Assessment of Antibacterial and Antifungal Properties and In Vivo Cytotoxicity of Peruvian Passiflora mollisima.

AIM: The antimicrobial activity of Passiflora mollissima is largely due to its aliphatic and aromatic hydrocarbons, terpenes, alkaloids, ketones, esters, alcohols, and lactones. The objective of this research was to evaluate the antimicrobial activity of the compounds in the Passiflora mollissima (Tumbo) fruit and leaves included also on cultured strains of the microorganisms Streptococcus mutans, Streptococcus oralis, Streptococcus sanguinis and Candida albicans. MATERIALS AND METHODS: Antimicrobial activity was analyzed by the disk diffusion method and evaluated in terms of their zones of inhibition. The cytotoxic activity of these compounds was determined in a bioassay involving sea urchin eggs (Tetrapygusniger) in which the inhibition percentage of artificially fertilized ovules was equivalent to their cytotoxic activity after 26 hours. RESULTS: This study demonstrated the antimicrobial activity of ethanolic Passiflora mollissima extract against the cultured strains of Streptococcus mutans, Streptococcus oralis, and Streptococcus sanguinis with zones of inhibition after the incubation period. CONCLUSION: There was no evidence of activity against the pathogenic Candida albicans, which demonstrates a null antifungal capacity of the evaluated extracts. However, cytotoxic activity was found, and the compounds led to the complete inhibition of 100% of sea urchin ovules after 26 hours of exposure. CLINICAL IMPORTANCE: The results of the study serve as a guide. in the industrial use of this natural resource "tumbo" due to the discovery of a potential medicine based on this plant that would be a great development in the field of antimicrobial therapies.

Metabolitos en las hojas de Erythroxylum coca Lam y Erithroxylum novogranatense (Morris) Hieron y evaluacón de sus propiedades biológicas mediante bioensayos / Metabolites detected in Erythroxylum coca Lam y Erythroxylum novogranatense (Morris) Hieron leaves and evaluation of their biological activities by bioessay

Objetivo: Realizar el screening fitoquímico y determinar la actividad antioxidante y antibacteriana de los flavonoides y alcaloides aislados de extractos etanólicos de las hojas de Erythroxylum coca Lam y Erythroxylum novogranatense (Morris) Hieron. Materiales y Métodos: La capacidad antioxidante de los flavonoides y alcaloides aislados fue evaluada mediante el ensayo de DPPH, utilizando como control positivo a la vitamina C, y la actividad antibacteriana mediante el cálculo del halo de inhibición (mm) ante S. epidermidis, P. aeruginosa, S. aureus y E. coli. Resultados: Los alcaloides de E. coca presentaron mayor actividad antibacteriana frente a S. epidermidis y P. aeruginosa, y los flavonoides de E. novogranatense tuvieron mayor actividad frente a S. epidermidis, P. aeruginosa y S. aureus. E. novogranatense mostroun EC50 de 271,20 µg/mL para extracto crudo y 250,29 µg/mL para flavonoides, mientras que E. coca presento un EC50 de 172,59 µg/mL para extracto crudo y 611,29 µg/mL para flavonoides. Conclusión: En ambas especies se identificaron alcaloides (cocaína y benzoilecgonina), flavonoides (chalconas y flavonoles), fenoles y carbohidratos. Los flavonoides de Erythroxylum novogranatense (Morris) Hieron, y el extracto de Erythroxylum coca Lam presentaron mayor actividad antioxidante. El extracto de Erythroxylum coca Lam mostro mayor actividad antibacteriana frente a S. aureus, S. epidermidis y E. coli, excepto para P. aeruginosa. Los alcaloides de Erythroxylum coca Lam, exhibieron buena actividad frente a S. epidermidis y P. aeruginosa; excepto para E. coli, donde Erythroxylum novogranatense (Morris) Hieron tuvo mejor desempeño. Los flavonoides aislados de Erythroxylum novogranatense (Morris) Hieron mostraron actividad frente a S. epidermidis.